Hub Genes in HSIL: Transcriptomic Profiles of 132 HPV-Positive Cervical Smears

A study by Mohamad Ammar Ayass, last updated in March 2026, presents transcriptomic data from 132 HPV-positive cervical Pap smear specimens. The dataset includes samples across four cytological stages (NILM, ASCUS, LSIL, HSIL) and integrates HPV genotyping and E6/E7 mRNA quantification to map molecular progression towards cervical cancer.

Use Cases

• Identify stage-specific gene expression signatures based on the described profiles for NILM, ASCUS, LSIL, and HSIL.
• Validate associations between E6/E7 mRNA levels and lesion severity based on the statistical result (X²=24.407, p=0.003) mentioned in the description.
• Analyze pathway crosstalk during malignant transformation based on the reported minimal overlap (OC < 0.07) between HSIL and earlier stages.

Strengths

• Integrates three data types: transcriptomic profiling, HPV genotyping, and E6/E7 mRNA quantification from a single study.
• Includes 132 clinical specimens across four distinct cytological stages (NILM, ASCUS, LSIL, HSIL).
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset is very small at 14.2 KB, indicating limited scope.

Provenance

Source

figshare, author Mohamad Ammar Ayass.

Collection Method

Integrative transcriptomic profiling of clinical Pap smear specimens.

Freshness

Last updated 2026-03-19 17:41:20.