Hub Genes in LSIL: Transcriptomic Profiles of 132 HPV-Positive Cervical Smears

132 HPV-positive cervical Pap smear specimens (NILM, ASCUS, LSIL, HSIL) were analyzed via integrative transcriptomic profiling, HPV genotyping, and E6/E7 mRNA quantification. The dataset, authored by Mohamad Ammar Ayass and last updated in March 2026, reveals stage-specific molecular signatures and pathway crosstalk. It is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Identify stage-specific gene expression signatures for cervical lesion classification based on transcriptomic profiles.
• Correlate E6/E7 mRNA expression levels with cytological abnormality severity as described in the study.
• Analyze pathway crosstalk and molecular discontinuity between HSIL and earlier stages of cervical dysplasia.
• Validate potential biomarkers for distinguishing transient HPV infections from high-risk lesions likely to progress.

Strengths

• Integrative data from 132 clinical specimens combining transcriptomics, HPV genotyping, and mRNA quantification.
• Stage-specific molecular signatures are identified for NILM, ASCUS, LSIL, and HSIL.
• Statistical association (p=0.003) reported between E6/E7 mRNA levels and lesion severity.

Limitations

• Dataset is very small at 13.5 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare, author Mohamad Ammar Ayass.

Collection Method

Integrative transcriptomic profiling of clinical Pap smear specimens.

Freshness

Last updated 2026-03-19 17:41:19; freshness should be verified.