IBAQ Proteomics Analysis of Host Cell Proteins in HA-3Dpol Precipitates

101.7 KB of Intensity-based Absolute Quantitation (IBAQ) proteomics data comparing host cell proteins in anti-HA precipitates from Huh-7.5 cells expressing HA-3Dpol-R468 or HA-3Dpol-K468 variants versus mock-transfected controls. The dataset was authored by Ichiro Misumi and last updated on May 11, 2026. It is available under a CC-BY-4.0 license as an XLSX file.

Use Cases

• Compare protein abundance levels based on IBAQ values between experimental conditions.
• Identify host cell proteins that differentially interact with HA-3Dpol protein variants.
• Validate protein interaction partners in precipitates from transfected cell lines.

Strengths

• Dataset is small (101.7 KB), facilitating quick download and inspection.
• Includes a control condition (mock transfected cells) for comparative analysis.
• Uses the IBAQ method for absolute protein quantitation, a recognized proteomics technique.

Limitations

• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The dataset's scope is limited to a specific experimental setup involving Huh-7.5 cells and HA-3Dpol variants.

Provenance

Source

figshare

Collection Method

Intensity-based Absolute Quantitation (IBAQ) proteomics analysis of anti-HA precipitates.

Freshness

Last updated 2026-05-11 17:40:14.