ITS2 Sequence-Structure Alignment for Haptophyte Phylogenetics

396 representative 18S rRNA sequences and 224 ITS2 sequences were used to study haptophyte phylogeny. Louisa Ridder applied a sequence-structure framework to enhance alignment and reduce artifacts. The dataset, last updated in March 2026, is a 30.8 KB subset focusing on ribosomal RNA secondary structure.

Use Cases

• Compare phylogenetic resolution between sequence-only and sequence-structure approaches based on the described methodology.
• Distinguish closely related haptophyte species based on ITS2 sequence variability mentioned in the description.
• Analyze conserved and variable features in 18S and ITS2 secondary structures based on comparative modeling results.
• Investigate the impact of taxon sampling on phylogenetic outcomes based on the reduction from over 40,000 sequences.
• Evaluate alignment challenges due to high sequence variability and length differences described for ITS2 data.

Strengths

• Includes 396 representative 18S rRNA sequences derived from over 40,000 available sequences.
• Compiles 224 ITS2 sequences for comparative analysis.
• Integrates secondary structure information, which reportedly improved alignment quality and reduced phylogenetic artifacts.

Limitations

• ITS2 data has limited taxon representation and alignment challenges due to high variability.
• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

Louisa Ridder via figshare

Collection Method

Taxon sampling reduced available sequences; comparative modeling and homology-based structure prediction were applied.

Freshness

Last updated 2026-03-19 17:23:05