Primer Sequences for Circular Polymerase Extension Reaction in Hepacivirus Research

A list of primers used to construct a reporter hepacivirus via circular polymerase extension reaction (CPER). Yasunori Akaike published this resource in 2026 to support research on surrogate models for hepatitis C virus. The dataset is a small 5.5 KB Excel file detailing the molecular tools for generating reporter viruses.

Use Cases

• Selecting primer sequences for constructing NRHV1 reporter viruses via CPER.
• Replicating the HiBiT reporter gene insertion into the NS5A domain III coding region.
• Designing experiments for in vitro antiviral screening using the provided primer list.
• Developing platforms for generating diverse hepacivirus reporter strains based on the described methodology.

Strengths

• Primer list is directly linked to a published virology study establishing a novel reporter virus platform.
• Dataset is openly shared under a CC-BY-4.0 license, facilitating reuse.
• Resource was updated in 2026, indicating recent relevance.

Limitations

• The dataset is very small at 5.5 KB, containing only a list of primers without experimental results or validation data.
• No sample data or column descriptions are provided, limiting immediate understanding of the data structure.
• The scope is narrow, focused solely on primer sequences for a specific CPER application.

Provenance

Source

Figshare, authored by Yasunori Akaike.

Collection Method

Primers were designed and used for circular polymerase extension reaction (CPER) to construct a reporter hepacivirus.

Freshness

Last updated in April 2026.