Primer Sequences for P-Like Fimbriae Study in Avian Pathogenic E. coli

Fariba Akrami's dataset contains lists of primers used to investigate P-like fimbriae in the avian pathogenic Escherichia coli strain QT598. The 5.5 KB XLS file was uploaded to figshare in April 2026. It supports a study on bacterial virulence factors in a natural turkey respiratory infection model.

Use Cases

• Designing PCR assays for plf gene detection using the provided primer sequences.
• Comparing primer sequences for P-like fimbriae with those for related adhesins like PapG II.
• Replicating the study's qRT-PCR methodology to measure plf gene expression in turkey lung tissue.

Strengths

• Primer sequences are directly linked to a published study on APEC strain QT598.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• Dataset is very small at 5.5 KB, containing only primer lists without experimental results.
• Scope is limited to a single bacterial strain (QT598) and a specific fimbrial operon.

Provenance

Source

Research by author Fariba Akrami, associated with a specific study on APEC.

Collection Method

Primers were designed and used for genetic manipulation and qRT-PCR in the described infection model.

Time Range

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Freshness

Last updated on figshare in April 2026.

Geography

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