Lysis Recovery Mutant Data from Mycobacterium Phages

Figshare hosts a dataset of lysis recovery mutants isolated from ΔlysF1b phages Girr and NormanBulbieJr. The data, 5.5 KB in size, was contributed by author Richard S. Pollenz and last updated in April 2026. It documents genetic and phenotypic changes in phages infecting Mycobacterium smegmatis.

Use Cases

• Correlate specific point mutations in the lysF1a gene with observed plaque size and lysis timing phenotypes.
• Analyze the impact of lysF1b gene deletion on burst size reduction and premature lysis triggering.
• Compare lysis phenotypes between single and double deletion mutants of lysF1a and lysF1b genes.
• Model the functional relationship between LysF1a and LysF1b proteins based on mutant complementation data.

Strengths

• Data includes quantified phenotypic measurements like ~65% burst size reduction.
• Documents specific genetic mutations mapped to defined protein regions (TMD1 or C-terminal).

Limitations

• Dataset is very small at 5.5 KB, indicating limited scope and sample count.
• Row count and specific column structure are unknown, limiting analytical depth.

Provenance

Source

Figshare, authored by Richard S. Pollenz.

Collection Method

Generated from laboratory experiments isolating and sequencing phage mutants.

Time Range

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Freshness

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Geography

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