Methylation Specific PCR Primers for Diabetic Endothelial Dysfunction Research

Eric Wang's dataset on figshare contains PCR primer information for studying epigenetic regulation in diabetic complications. The data, last updated in May 2026, is a 5.5 KB XLS file. It originates from experiments using human retinal and cardiac microvascular endothelial cells treated with epigenetic inhibitors.

Use Cases

• Designing PCR experiments based on the provided primer sequences for methylation studies.
• Comparing epigenetic regulatory mechanisms between cardiac and retinal endothelial cells based on the described experimental context.
• Investigating the role of DNA methylation versus histone methylation in glucose-induced miR-9 inhibition.
• Analyzing the interplay between long non-coding RNAs ZFAS1 and MALAT1 in retinal endothelial dysfunction.

Strengths

• Data is shared under a permissive CC-BY-4.0 license.
• File size is 5.5 KB, indicating a focused and likely manageable dataset.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The description is detailed but does not specify the exact primer sequences or experimental parameters contained in the file.

Provenance

Source

figshare

Collection Method

Likely generated from laboratory experiments involving cell culture, siRNA transfection, and treatment with epigenetic inhibitors.

Freshness

Last updated 2026-05-21 17:27:05; freshness should be verified.