32.7 KB of expression ratios for candidate regulatory sequences (CRS) in two human liver cell lines. The data, authored by Kyle Leix and last updated in March 2026, quantifies activity by comparing cDNA to genomic DNA barcode counts across five independent replicates. Ratios are normalized to negative control sequences for each sample.
Use Cases
- Identify particularly strong regulatory elements based on normalized expression ratios.
- Compare gene expression activity between HuH7 and HepG2 cell lines.
- Validate candidate cis-regulatory sequences (CRS) mentioned in the description.
- Assess the impact of sequence variation on 5'UTR function.
Strengths
- Includes data from 5 independent biological replicates for each cell line.
- Ratios are normalized to negative controls within each sample.
- Data is openly licensed under CC-BY-4.0.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
- The dataset is small at 32.7 KB, indicating limited scope.
Provenance
- Source
- figshare
- Collection Method
- Expression quantified from aggregate barcode counts in cDNA relative to genomic DNA.
- Time Range
- null
- Freshness
- Last updated 2026-03-17 17:32:08; freshness should be verified.
- Geography
- null