Neighborhood Relationships Between 77 Human Lymph Node Cell Types

78-marker antibody panel data from 19 healthy human lymph nodes, classified by the MILAN staining method and BRAQUE pipeline. The dataset spatially maps 77 cell types, including 27 unique T-cell subsets, and reveals novel cellular neighborhoods. Author Maddalena M. Bolognesi published this proteomic interrogation on figshare in April 2026.

Use Cases

• Analyzing spatial cell-cell interactions based on statistically controlled pairwise neighborhood analysis.
• Classifying T-cell subsets based on expression profiles of TCF7 and co-inhibitory receptors.
• Mapping novel B-cell niches based on CD5 and TCF7 expression and spatial distribution.
• Identifying landscape organization of lymph nodes based on high-dimensional proteomic data.

Strengths

• Data is derived from 19 human lymph nodes free of pathology, providing a healthy baseline.
• Classification encompasses 77 cell types using a 78-marker antibody panel.
• Analysis includes 27 unique CD4 and CD8 T-cell subsets defined by multiple markers.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (87.7 KB), indicating limited scope or highly summarized data.

Provenance

Source

figshare

Collection Method

Hyperplexed cyclic staining method (MILAN) and analytical bioinformatic pipeline (BRAQUE) applied to tissue samples.

Time Range

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Freshness

Last updated 2026-04-21 17:54:13; freshness should be verified.

Geography

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