Mycobacterium tuberculosis RB-TnSeq Library Screens for 95 Conditions

A pooled random barcode transposon-site sequencing (RB-TnSeq) library was generated for Mycobacterium tuberculosis to enable high-throughput genetic screening. The dataset includes results from 95 screens on carbon sources, nitrogen sources, stressors, and antibiotics, uncovering 187 novel phenotypes for the elusive pe/ppe gene family. This resource, created by Kayla M. Dinshaw and shared under CC-BY-4.0, facilitates hypothesis generation about uncharacterized genes in Mtb metabolism and stress resistance.

Use Cases

• Identify genes essential for nutrient utilization based on screens of carbon and nitrogen sources.
• Investigate genetic determinants of antibiotic resistance based on screening results for pretomanid and other antibiotics.
• Analyze the function of the pe and ppe gene family based on 187 novel phenotypes uncovered across 37 genes.
• Study metabolic pathways like lactate and propionate utilization based on proposed roles for the ESX-5 secretion system and Nuo dehydrogenase.

Strengths

• Library uses a unique 20-nucleotide barcode for high-throughput screening, bypassing laborious traditional TnSeq protocols.
• Results are derived from 95 distinct genetic screens across a diverse array of conditions.
• The study uncovered 187 novel phenotypes for the poorly understood pe/ppe gene family.

Limitations

• The dataset is small at 11.6 KB, indicating limited scope or summary-level data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analyses.

Provenance

Source

Kayla M. Dinshaw via figshare

Collection Method

Generated from a pooled random barcode transposon-site sequencing (RB-TnSeq) library in Mycobacterium tuberculosis, followed by 95 genetic screens.

Freshness

Last updated 2026-04-15 18:00:14; freshness should be verified.