Oligonucleotide Primer Sequences and GenBank Accessions for Echinococcus

Oligonucleotide primer sequences for whole-mount in situ hybridization (WISH) and quantitative reverse transcription PCR (qRT-PCR) experiments are provided, along with associated GenBank accession numbers. The dataset was authored by Ruth Herrmann and is available under a CC BY 4.0 license. Its small size of 14.5 KB indicates a focused collection of primer and accession data.

Use Cases

• Designing PCR assays using the provided oligonucleotide primer sequences for gene expression studies.
• Validating gene targets in Echinococcus multilocularis research by cross-referencing the listed GenBank accession numbers.
• Supporting whole-mount in situ hybridization (WISH) experiments with the included primer sequences for probe synthesis.

Strengths

• Data is openly shared under a permissive CC BY 4.0 license.
• Primer sequences are specifically curated for techniques like WISH and qRT-PCR, providing direct experimental utility.
• The dataset includes GenBank accession numbers, enabling direct linkage to public sequence databases.

Limitations

• The dataset is very small at 14.5 KB, containing a limited number of primer sequences and accessions.
• The specific row count and column structure are unknown, making it difficult to assess the dataset's full scope programmatically.
• The data is focused on a specific organism (Echinococcus multilocularis), limiting broader applicability.

Provenance

Source

Ruth Herrmann via figshare.

Collection Method

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Time Range

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Freshness

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Geography

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