James A. Sawitzke's dataset on figshare characterizes parameters for in vivo linear DNA assembly mediated by RecET and λ Red recombination systems. The data, last updated in April 2026, likely contains experimental results on assembly efficiency, including the impact of Exonuclease I deletion and comparisons to in vitro methods. The file is a 14.9 KB XLSX spreadsheet.
Use Cases
- Benchmarking in vivo assembly efficiency based on terminal homology length described in the study
- Comparing RecET and λ Red recombination system performance as detailed in the description
- Analyzing the impact of Exonuclease I gene deletion on assembly accuracy and error rates
- Evaluating multi-fragment assembly feasibility for constructing functional plasmids from six segments
Strengths
- Data is associated with a published scientific characterization study
- File size of 14.9 KB suggests a focused, manageable dataset
- Released under a permissive CC-BY-4.0 license
Limitations
- Row count and column-level documentation are absent; field semantics must be inferred after download
- Description metadata is limited; actual data quality requires manual inspection after download
Provenance
- Source
- James A. Sawitzke via figshare
- Collection Method
- Likely contains experimental results from genetic engineering assays
- Time Range
- null
- Freshness
- Last updated 2026-04-03 17:49:21; freshness should be verified
- Geography
- null