Human Lymph Node Spatial Proteomics with 77 Cell Types

Maddalena M. Bolognesi's dataset provides a high-dimensional proteomic map of 19 normal human lymph nodes, classifying 77 cell types using a 78-marker antibody panel and the MILAN staining method. The data includes spatial allocation for T, B, innate immune, and stromal cells, revealing novel interactions and landscape niches. It was last updated on April 21, 2026.

Use Cases

• Analyzing spatial distribution of T-cell subsets based on TCF7 and co-inhibitory receptor expression profiles.
• Mapping novel B-cell types and their nodal niches based on CD5 and TCF7 expression.
• Studying statistically controlled pairwise neighborhood interactions between immune and stromal cells.
• Investigating the organization of type 2 conventional dendritic cells within paracortical aggregates.

Strengths

• Data is derived from 19 human lymph nodes free of pathology, providing a normal tissue baseline.
• Classification encompasses 77 distinct cell types using a 78-marker antibody panel.
• Includes spatial distribution data for CD4 and CD8 T-cells across 27 unique subsets.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 32.3 KB, indicating limited scope.

Provenance

Source

Maddalena M. Bolognesi via figshare

Collection Method

Hyperplexed cyclic staining method (MILAN) and analytical bioinformatic pipeline (BRAQUE) applied to 19 human lymph nodes.

Time Range

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Freshness

Last updated 2026-04-21 17:54:15; freshness should be verified.

Geography

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