PCR and Sequencing Primers for Xenopus Parasite Study in Nigeria

Primer sequences used in a study of host-parasite biodiversity in African clawed frogs across Nigeria. The dataset, authored by Emmanuela U. Anele and last updated in May 2026, is a 10.8 KB Excel file containing primers for Sanger sequencing of five Xenopus species and thirteen helminth parasite species.

Use Cases

• Replicate molecular identification of Xenopus species based on the primer sequences.
• Design follow-up studies on helminth parasite diversity using the provided primer sets.
• Compare host specificity and tissue tropism of parasites by applying the described molecular tools.
• Conduct phylogenetic analysis of Nigerian amphibian parasites using the Sanger sequencing approach.

Strengths

• Primers are linked to a specific study covering five Xenopus species and thirteen helminth species across multiple ecological zones in Nigeria.
• Data is shared under a permissive CC-BY-4.0 license, facilitating reuse.
• The 10.8 KB size indicates a focused, directly usable list of primer sequences.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset's scope is limited to the primers used in a single study, not a comprehensive primer database.

Provenance

Source

figshare, author Emmanuela U. Anele

Collection Method

Primers were used for morphological and Sanger sequencing characterization of hosts and parasites.

Freshness

Last updated 2026-05-19 17:38:23; freshness should be verified.

Geography

Several ecological zones of Nigeria