Primary and Secondary Antibody Table for 19 Human Lymph Nodes

A 78-marker antibody panel used to classify 77 cell types in 19 normal human lymph nodes via the MILAN hyperplexed staining method and BRAQUE bioinformatic pipeline. The dataset, created by Maddalena M. Bolognesi and last updated in April 2026, provides a high-dimensional proteomic map with spatial allocation of T, B, innate immune, and stromal cells. It is a small, 75.7 KB Excel file.

Use Cases

• Classifying T-cell subsets based on expression profiles of TCF7 and co-inhibitory receptors.
• Identifying novel B-cell types based on CD5 and TCF7 expression patterns.
• Mapping spatial niches and cell-cell interactions via statistically controlled pairwise neighborhood analysis.
• Locating specific immune cell populations, such as CD27+ mature B-cells and type 2 conventional dendritic cells, within lymph node structures.

Strengths

• Provides a high-resolution spatial proteomic map of 19 normal human lymph nodes.
• Classifies 77 distinct cell types using a 78-marker antibody panel.
• Identifies 27 unique CD4 and CD8 T-cell subsets based on specific protein expression.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (75.7 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Hyperplexed cyclic staining method (MILAN) and analytical bioinformatic pipeline (BRAQUE) applied to tissue samples.

Time Range

The data collection date is not specified.

Freshness

Last updated 2026-04-21 17:54:05.

Geography

Human lymph nodes; specific geographic origin is not stated.