Primer and Probe Sequences for BVDV and E. coli K99 Detection

Primer and probe sequences for detecting Bovine Viral Diarrhea Virus (BVDV) and Escherichia coli K99 gene. The sequences were designed based on the 5′-UTR of BVDV (GenBank ON901784.1) and the conserved K99 gene region of E. coli (GenBank M35282.1). Wenxin Zhu authored this dataset, last updated on 2026-05-15.

Use Cases

• Designing a multiplex qPCR assay based on the provided primer and probe sequences.
• Detecting Bovine Viral Diarrhea Virus based on the 5′-UTR region sequences.
• Detecting Escherichia coli K99 gene based on the conserved region sequences.
• Validating in-house PCR protocols against published primer sequences.

Strengths

• Primer sequences are linked to specific GenBank accession numbers (ON901784.1, M35282.1).
• Dataset is openly licensed under CC-BY-4.0.
• File format is XLS, which is widely compatible with spreadsheet software.

Limitations

• Dataset is very small (5.5 KB), indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Primers were designed based on specific GenBank sequences.

Freshness

Last updated 2026-05-15 17:29:18; freshness should be verified.