Primer Sequences for RNA-Dependent RNA Polymerase Activity Studies

Primer sequences supporting research on the RNA-dependent RNA polymerase (RdRP) activity of DNA-dependent RNA polymerases. The dataset, 10.2 KB in size, was authored by Jie Hao and last updated on April 30, 2026. It provides evidence for the reorganization of polymerase II for transcribing the plant viroid PSTVd, aided by a splicing variant of transcription factor IIIA.

Use Cases

• Designing PCR primers for experiments on PSTVd transcription based on the described primer sequences.
• Validating structural predictions of the Pol II/TFIIIA-7ZF/PSTVd complex generated by AlphaFold3.
• Investigating the role of specific polymerase subunits (Rpb4/5/6/7/9) in RNA template recognition as described in the study.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting broad reuse.
• Data is associated with a specific, recent research update from April 30, 2026.
• The 10.2 KB size indicates a focused and likely manageable set of sequences.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The description is highly specific to a niche biological process; generalizability is limited.

Provenance

Source

Jie Hao via figshare

Collection Method

Likely generated from experimental research on PSTVd transcription in plants.

Freshness

Last updated 2026-04-30 17:51:49; freshness should be verified.