Primer Sequences for Real-Time PCR Analysis of Cadmium Toxicity in Freshwater Crabs

Primer sequences used for real-time PCR analysis in a study of cadmium toxicity in freshwater crabs. The dataset is 5.5 KB in size, stored in an XLS file, and was authored by Jianxia Liu. It was last updated on April 22, 2026, and is shared under a CC-BY-4.0 license.

Use Cases

• Designing PCR assays based on the provided primer sequences for genes like cytochrome P450 (CYP450) and heat shock proteins (Hsp21, Hsp27, Hsp70).
• Analyzing gene expression patterns related to protein detoxification and folding in response to heavy metal exposure.
• Correlating molecular data with histological observations of cell injury in gill tissue.
• Studying the activation of the DAG-PKC/IP3-Ca2+ signaling pathway induced by cadmium at the genetic level.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.
• File size is 5.5 KB, indicating a focused and likely manageable dataset.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

figshare, authored by Jianxia Liu.

Collection Method

Likely generated from laboratory experiments involving cadmium exposure and PCR analysis.

Time Range

The study involved a 4-day exposure period, but the dataset's creation date is not specified.

Freshness

Last updated 2026-04-22 17:31:36; freshness should be verified.

Geography

The study organism is the freshwater crab Sinopotamon henanense; specific geographic origin is not stated.