Pleurotus Ostreatus Mating Type Primer Sets and Genomic Analysis

Primer sets and genomic data for characterizing the multiallelic mating-type loci in the edible oyster mushroom Pleurotus ostreatus. Yi-Yun Lee developed this resource, which includes analysis of 12 haplotypes identifying 11 A and 12 B alleles. The dataset was last updated in April 2026.

Use Cases

• Design PCR primers for mating type detection in Pleurotus ostreatus using the provided primer sequences.
• Analyze allele diversity at the matA and matB loci across 12 identified haplotypes.
• Compare genomic similarity between the congenic strain PC9.15 and the parental PC9 strain, focusing on the introgressed mating-type loci.
• Validate genome assembly contiguity and quality for the PC9.15 strain generated through 10 rounds of backcrossing.

Strengths

• Identifies 11 A and 12 B alleles from 12 analyzed haplotypes, confirming multiallelic nature.
• Genomes of compared strains PC9 and PC9.15 are reported as 99% similar, providing a clear baseline.
• Dataset is associated with a high-quality and contiguous genome assembly for the congenic strain PC9.15.

Limitations

• Dataset is very small at 9.1 KB, indicating limited scope, likely containing only primer lists and summary tables.
• Sample size for allele identification is based on an unspecified number of strains beyond the core PC9 and PC15 pair.
• No raw sequencing reads or full genomic alignments are included, only derived results and primers.

Provenance

Source

Research by Yi-Yun Lee, published on figshare.

Collection Method

Genomic sequencing, assembly, and bioinformatic analysis of mating-type loci; primer design.

Freshness

Last updated in April 2026.