Primer Sequences for MeRIP-qPCR in Glaucoma Retinal Injury Study

A figshare dataset from 2026 by Yahong Li provides primer sequences used for methylated RNA immunoprecipitation quantitative PCR (MeRIP-qPCR). The 5.5 KB Excel file supports a study on the role of the NSUN4 methyltransferase in retinal cell survival and the Sonic Hedgehog signaling pathway in glaucoma models. The data is licensed under CC-BY-4.0.

Use Cases

• Validating m5C RNA methylation levels on specific transcripts like Shh, Gli1, and Gli2 based on the described MeRIP-qPCR methodology.
• Replicating experimental protocols for studying excitotoxic injury in R28 retinal cell lines.
• Investigating correlations between NSUN4 expression and clinical samples from primary open-angle glaucoma patients as mentioned in the study.

Strengths

• Dataset is small (5.5 KB), facilitating quick download and inspection.
• Associated with a specific, peer-reviewed research study mechanism described in the metadata.
• Available under a permissive CC-BY-4.0 license for reuse.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (5.5 KB), indicating limited scope, likely containing only primer sequences.

Provenance

Source

figshare, author Yahong Li.

Collection Method

Likely generated as part of a laboratory study using MeRIP-seq and RNA-seq on NMDA-induced retinal injury and R28 cell line models.

Freshness

Last updated 2026-04-15 17:46:54; freshness should be verified.