Primers and probe pairs for a multiplex qPCR assay designed to detect Aspergillus spp., Histoplasma spp., and Pneumocystis jirovecii DNA in sputum. The assay was validated and applied to 273 stored sputum samples from adult patients in The Gambia presenting with tuberculosis-like symptoms. Zackary Salem-Bango published this dataset on figshare in April 2026.
Use Cases
- Validate multiplex qPCR assays for fungal pathogen detection based on the described primer/probe sequences.
- Benchmark diagnostic sensitivity and specificity for Aspergillus, Histoplasma, and Pneumocystis jirovecii based on the assay's reported performance.
- Support surveillance studies of fungal colonization in tuberculosis-symptomatic populations based on the study's application in Gambian sputum samples.
Strengths
- The multiplex assay demonstrated high sensitivity and specificity, detecting as few as ten DNA copies per reaction.
- The dataset is associated with a study analyzing 273 sputum samples from a defined patient cohort in The Gambia.
- The assay represents the first molecular detection of Aspergillus spp. and Pneumocystis jirovecii in adults in The Gambia.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
- The dataset is very small (5.5 KB), indicating limited scope likely containing only primer/probe sequences.
Provenance
- Source
- figshare
- Collection Method
- Primers and probe pairs were developed and validated for a multiplex qPCR assay.
- Freshness
- Last updated 2026-04-24 17:45:10; freshness should be verified.
- Geography
- The Gambia