Primers for RT-qPCR: Noncoding RNA Expression in Heat Acclimation Cardioprotection

Nine core molecular candidates, including miR-196c-5p and Rffl, were identified in a transcriptomic study of heat acclimation-mediated cardioprotection in rat models. The dataset, 9.5 KB in size, was uploaded by LiJun Fan to figshare and last updated on 2026-04-29. It delineates two central ceRNA regulatory axes potentially coordinating mitochondrial integrity and inflammatory signaling.

Use Cases

• Validate RT-qPCR assays for the nine identified molecular candidates (e.g., miR-196c-5p, Rffl, Rassf1) based on the transcriptomic profiling results.
• Investigate the MSTRG.6276.3–miR-196c-5p–Rffl ceRNA regulatory axis mentioned in the description for its role in apoptotic regulation.
• Analyze the immunomodulatory effects suggested by cytokine level changes (IL-1β decreased 24.36%, IL-6 increased 25.67%) in the context of heat stress.
• Study the potential coordination of mitochondrial integrity and hypoxic adaptation through the regulatory networks described.

Strengths

• Identifies nine specific molecular candidates (e.g., miR-196c-5p, Rffl, Cxcl2) and two ceRNA regulatory axes from the analysis.
• Includes specific quantitative findings from the study, such as IL-1β decreasing by 24.36% and IL-6 increasing by 25.67%.
• Released under a permissive CC-BY-4.0 license, facilitating reuse and sharing.

Limitations

• Row count is unknown, which may limit suitability assessment for statistical analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (9.5 KB), indicating a limited scope, likely containing only primer sequences or a summary list.

Provenance

Source

LiJun Fan via figshare

Collection Method

Derived from high-throughput sequencing and bioinformatic analysis of myocardial tissues from rat models of heat stress and heat acclimation preconditioning.

Freshness

Last updated 2026-04-29 17:40:30; freshness should be verified.