Primers for RT-qPCR: Hub Genes for Spinal Cord Injury Macrophage Polarization

A small dataset of primers for RT-qPCR validation of three hub genes identified in a spinal cord injury (SCI) study. The dataset was created by Xiaowei Zha and last updated on May 4, 2026. It contains results from a study intersecting differentially expressed genes with macrophage polarization-related genes to identify potential therapeutic targets.

Use Cases

• Validating the expression of hub genes Soat1, Comt, and Myo1f in spinal cord injury models based on the RT-qPCR primers described.
• Investigating immune-related pathways in spinal cord injury based on the functional enrichment analysis mentioned in the description.
• Identifying potential drug candidates for modulating macrophage polarization based on the in silico prediction of 37 small molecules.
• Analyzing immune cell infiltration differences in spinal cord injury based on the 11 immune gene sets correlated with hub genes.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.
• The underlying study employed multiple machine learning algorithms (LASSO, RF, XGBoost) for gene selection.
• Findings were validated using single-cell RNA sequencing and RT-qPCR as described.

Limitations

• Dataset is very small at 5.5 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Generated from a bioinformatics study intersecting gene lists and applying machine learning algorithms.

Time Range

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Freshness

Last updated 2026-05-04 17:30:57; freshness should be verified.

Geography

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