Primers for qRT-PCR of 15 Selected Genes in Rice Glume Closure Study

Primers of qRT-PCR for the 15 selected genes is a dataset by Youming Huang, shared on figshare under a CC-BY-4.0 license and last updated in May 2026. The 5.5 KB XLS file contains primer sequences for genes involved in ABA synthesis and signaling in rice. The data supports a study on abscisic acid's role in regulating glume closure and hybrid seed yield.

Use Cases

• Designing qRT-PCR experiments to validate RNA-seq results based on the 15 selected genes mentioned in the description
• Studying the role of ABA synthesis genes (e.g., NCED, AAO) and signaling families (PYR/PYL, SnRK2, ABF, PP2C) in rice spikelet development
• Investigating molecular mechanisms underlying glume closure in fertile and sterile rice varieties described in the associated research
• Supporting hybrid breeding strategies that apply MeJA and ABA treatments to improve seed setting rates

Strengths

• Primer sequences are provided for 15 specific genes identified in a described RNA-seq analysis.
• Dataset is directly linked to a detailed experimental study involving multiple rice varieties and hormone treatments.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 5.5 KB, indicating limited scope.

Provenance

Source

Youming Huang

Collection Method

Likely derived from RNA sequencing analysis of rice lodicules during spikelet opening and closing phases.

Freshness

Last updated 2026-05-21 07:33:59; freshness should be verified.