Primers for PCR Amplification of Citrus Greening Pathogen in Nepal

Richa Giri's dataset contains primers used for PCR amplification in a 2026 study of citrus greening disease (huanglongbing) in Nepal. The study analyzed 1,026 leaf samples from 13 districts across six provinces, identifying 255 positive cases. Generated sequence data was deposited in GenBank and used for phylogenetic analysis.

Use Cases

• Replicate PCR-based diagnosis of HLB based on the described primer set Las606/LSS.
• Compare genetic sequences from Nepal with global HLB strains based on the deposited GenBank sequences.
• Design regional surveillance strategies based on the reported prevalence data across 13 districts.
• Validate computational phylogenetic methods based on the Sanger sequencing results from five samples.

Strengths

• Primer set validated with positive and negative controls in every PCR run.
• PCR assays were performed in triplicate to ensure consistency and reproducibility.
• Study includes 1,026 samples providing a substantial basis for prevalence assessment.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 5.5 KB, indicating limited scope.

Provenance

Source

Richa Giri via figshare

Collection Method

Primers used for conventional PCR amplification targeting the 16S rRNA gene.

Freshness

Last updated 2026-05-27 17:37:43; freshness should be verified.

Geography

Thirteen districts across six provinces in Nepal.