Fanqiang Zeng published a dataset of primers used for quantitative real-time PCR analysis on figshare in April 2026. The dataset supports a study investigating calcitriol's therapeutic effects on type 2 diabetes via endoplasmic reticulum stress pathways in a high glucose-induced MIN6 cell model. The dataset is 5.5 KB in size and is available in XLS format.
Use Cases
- Validate predicted gene targets STAT3, HSP90AA1, MAPK1, and PIK3R1 based on the study's network pharmacology analysis.
- Assess mRNA expression changes in MIN6 pancreatic β-cells under high glucose conditions.
- Correlate qRT-PCR primer sequences with protein level changes measured by western blot.
- Design follow-up experiments on endoplasmic reticulum stress pathways in type 2 diabetes.
Strengths
- Dataset is licensed under CC-BY-4.0, allowing open reuse.
- The associated study describes a clear experimental validation pipeline combining network pharmacology, molecular docking, and cell model assays.
- The dataset is small (5.5 KB), facilitating quick download and inspection.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
- The dataset is highly specific to a single cell model (MIN6) and four target genes.
Provenance
- Source
- Fanqiang Zeng
- Collection Method
- Likely generated as part of a laboratory study using high glucose-induced MIN6 cells.
- Freshness
- Last updated 2026-04-16 17:32:29; freshness should be verified.