qRT-PCR Primers for Botrytis Pseudocinerea Pathogenicity Study

Victor Coca-Ruiz provides a primer list used for qRT-PCR in a 2026 study comparing the fungal pathogens Botrytis pseudocinerea and Botrytis cinerea. The 5.5 KB Excel file contains sequences for analyzing gene expression related to toxin production and host specialization.

Use Cases

• Design qRT-PCR assays targeting specific genes like Bcboa6 or Bcboa9 for expression analysis in Botrytis species.
• Verify primer sequences for studying the botcinin polyketide pathway in fungal pathogenicity experiments.
• Cross-reference primer data with gene expression results to validate the loss of the botrydial sesquiterpene pathway in B. pseudocinerea.

Strengths

• Primers are directly linked to a published molecular study on fungal pathogenicity and metabolic pathways.
• Data is provided in a standard XLS format, facilitating easy integration into laboratory workflows.
• Dataset is clearly attributed to author Victor Coca-Ruiz with a CC BY 4.0 license for open reuse.

Limitations

• The dataset is very small at 5.5 KB, containing only primer sequences without accompanying gene expression results.
• Lacks metadata on primer efficiency, annealing temperatures, or validation details typically required for experimental replication.
• Scope is limited to the specific isolate VD165 and comparative strain B05.10 used in the original study.

Provenance

Source

Victor Coca-Ruiz via figshare.

Collection Method

Primers were designed and used for qRT-PCR in a study of Botrytis pseudocinerea isolate VD165.

Time Range

Associated with the 2026 publication date.

Freshness

Last updated on 2026-03-24.