Primers for Burkholderia cepacia Detection in Goats, Bangladesh

Eight isolates from 40 goat blood samples in Bangladesh were identified as positive for the Burkholderia cepacia complex (Bcc), a first detection in animals in the country. The dataset contains primers used for molecular identification via PCR, Sanger sequencing, and phylogenetic analysis. It was authored by Abdullah Al Mamun and last updated in May 2026.

Use Cases

• Designing PCR assays for Burkholderia cepacia complex detection based on the listed primer sequences.
• Comparative phylogenetic analysis of Bcc strains based on the reference sequences from India, Japan, and China mentioned in the study.
• Assessing zoonotic risk and epidemiology of Bcc in livestock populations based on the serological and molecular test results described.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse and redistribution.
• Study provides specific results, including 8 positive isolates from 30 symptomatic goats and phylogenetic confirmation.
• Focus on a first-time detection event in Bangladesh adds regional specificity.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 5.5 KB, indicating limited scope.

Provenance

Source

figshare, authored by Abdullah Al Mamun.

Collection Method

Primers used in a study involving blood sample collection, serological tests (GRDTK, ELISA), and molecular tests (PCR, sequencing).

Time Range

Study period not specified; dataset updated in 2026.

Freshness

Last updated 2026-05-22 17:40:31; freshness should be verified.

Geography

Goat samples collected in Bangladesh.