Primers for Cryptococcus Neoformans CO2 Tolerance Genetic Screen

301 deletion mutants of Cryptococcus neoformans showed altered fitness under high CO2 conditions in a near genome-wide screen of 4,692 mutants. The dataset, authored by Laura C. Ristow and shared on figshare, contains primers used in the study. It was last updated on May 5, 2026.

Use Cases

• Designing PCR primers for validating deletion mutants based on the genetic screen results.
• Investigating the genetic basis of CO2 tolerance in fungal pathogens based on the identified mutant list.
• Studying the TOR-Ypk1 signaling axis in fungal stress response as described in the associated research.
• Analyzing the link between capsule formation, carbon metabolism, and virulence traits in Cryptococcus neoformans.

Strengths

• Primers are associated with a specific genetic screen of 4,692 deletion mutants.
• The underlying study identified 301 mutants with altered CO2 tolerance, providing a clear biological context.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• The dataset is very small at 11.4 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Primers used in a laboratory-based competitive growth assay genetic screen.

Freshness

Last updated 2026-05-05 17:37:26; freshness should be verified.