Primer Sequences for Listeria SigB Operon Mutation Study

Primers used in a study investigating genetic plasticity in the general stress response of the food-borne pathogen Listeria monocytogenes. Jialun Wu published this supporting data in 2026 on figshare. The 9.5 KB XLS file contains oligonucleotide sequences designed to target specific mutations within the sigB operon.

Use Cases

• Designing PCR assays to detect rsbW start codon mutations (ATG to ATA/ATT) identified in acid-resistant derivatives.
• Replicating the translational reporter assay by using the provided primer sequences to construct gene fusions.
• Investigating genetic clade-specific start codon usage by aligning primer sequences to conserved genes in L. monocytogenes genomes.
• Studying the impact of rsbX N77K and rsbU Q317* mutations on SigB activity through targeted mutagenesis using these primers.

Strengths

• Primers are directly linked to a published study analyzing 60,692 L. monocytogenes genomes.
• Data is openly licensed under CC-BY-4.0 for reuse and verification.

Limitations

• The dataset is very small at 9.5 KB, containing only primer sequences without associated experimental results.
• The exact number of primer sequences (rows) is unknown, limiting assessment of scope.
• Lack of sample data or column descriptions prevents immediate understanding of the data structure.

Provenance

Source

Jialun Wu, via figshare.

Collection Method

Primers were designed and used for sequencing, mutagenesis, and reporter assays in the associated research.

Time Range

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Freshness

Last updated in April 2026.

Geography

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