Yu Chi published a dataset of primers used in a study of phosphopantetheinyl transferase (PPTase) in Pseudomonas plecoglossicida NB2011. The dataset, available as an XLS file of 5.5 KB, was uploaded to figshare and last updated on March 19, 2026. The study investigated the enzyme's role in virulence, fatty acid synthesis, and bacterial survival.
Use Cases
- Designing primers for homologous recombination based on the primer sequences.
- Analyzing gene sequence similarity based on the alignment data mentioned.
- Studying bacterial growth phenotypes based on the mutant strain comparisons.
- Investigating virulence factor expression based on the RNA-seq results referenced.
Strengths
- Dataset is openly licensed under CC-BY-4.0.
- File size is 5.5 KB, indicating a compact and focused dataset.
- Last updated date is explicitly provided (2026-03-19).
Limitations
- Row count is unknown, which may limit suitability assessment.
- Column-level documentation is absent; field semantics must be inferred after download.
- Description metadata is limited; actual data quality requires manual inspection after download.
Provenance
- Source
- Yu Chi
- Collection Method
- Primers used for constructing a deletion mutant via double homologous recombination.
- Freshness
- Last updated 2026-03-19 17:31:21; freshness should be verified.