Otostegia Fruticosa Effects on Triple-Negative Breast Cancer Cells

An IC50 value of 23 µg/mL was measured for the cytotoxic effect of Otostegia fruticosa ethanol extract on MDA-MB-231 triple-negative breast cancer cells. Abdulrahman Alasmari authored this dataset, which likely contains results from MTT assays, fluorescence staining, and gene expression analysis for apoptotic and metastatic markers. The dataset was last updated on 2026-05-26.

Use Cases

• Analyze cytotoxic potency based on IC50 values mentioned in the description
• Model apoptosis induction based on reported changes in caspase, Bcl2, Bax, and Bid gene expression
• Study metastasis inhibition based on reported downregulation of MMP-9 and OPN proteins
• Correlate reactive oxygen species (ROS) generation with cell viability reduction as evidenced by DCFH-DA staining

Strengths

• Dataset includes a specific IC50 value of 23 µg/mL for cytotoxicity
• Results from multiple assays (MTT, morphological analysis, fluorescence staining, Trypan blue, invasion, migration) are likely compiled
• Gene expression analysis covers key apoptotic (Caspase-3,8,9, Bcl2, Bax, Bid, Cytochrome c, PTEN) and metastatic (MMP-9) markers

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is very small at 9.5 KB, indicating limited scope

Provenance

Source

figshare

Collection Method

Experimental study employing MTT, morphological analysis, fluorescence staining, Trypan blue exclusion, invasion, migration assays, qRT-PCR, immunofluorescence, and Western blot analysis.

Freshness

Last updated 2026-05-26 17:29:09; freshness should be verified