qPCR Primer Sequences for Murine Intestinal Organoid Infection Studies

9.5 KB Excel file containing qPCR primer sequences used in a study of host-bacterial interactions in primary murine intestinal epithelial cells. The dataset supports research on NLRC4-mediated responses to Shigella flexneri infection using ileal organoids from wild-type and Nlrc4-deficient mice. It was authored by Marry Nissan and shared under a CC BY 4.0 license.

Use Cases

• Design qPCR assays targeting genes identified in transcriptional analysis of infected Nlrc4-/- organoids.
• Validate gene expression changes related to the enteroendocrine cell (EEC) lineage marker enrichment observed during infection.
• Replicate experiments measuring host response to bacteria-free S. flexneri supernatants and the type three secretion system (T3SS).

Strengths

• Primer sequences are directly linked to a specific, peer-reviewed experimental model using murine ileal organoids.
• Dataset is shared under a permissive CC BY 4.0 license, enabling broad reuse.
• Associated study provides detailed biological context for the NLRC4 and S. flexneri host-pathogen interaction.

Limitations

• The dataset is very small at 9.5 KB, indicating limited scope, likely containing only primer sequences without accompanying expression data.
• No sample data or column descriptions are available, making initial assessment of data structure difficult.
• The experimental focus is narrow, centered on a specific murine model and bacterial pathogen, limiting generalizability.

Provenance

Source

Figshare, authored by Marry Nissan.

Collection Method

Primer sequences generated for qPCR validation of transcriptional analysis from murine ileal organoid infection experiments.

Freshness

Last updated March 2026.