qRT-PCR Primer Sequences for Pulmonary Arterial Hypertension Hub Gene Validation

A 5.5 KB XLS file containing primer sequences for quantitative real-time PCR validation of immune-associated hub genes in pulmonary arterial hypertension (PAH). The dataset was authored by Xitong Yang and last updated on May 22, 2026. It supports a study that integrated transcriptomic data from the Gene Expression Omnibus (GEO) and a murine model to identify genes like BCLAF1, CDC5L, SMARCA5, and ASH1L.

Use Cases

• Designing qRT-PCR experiments to validate hub gene expression based on the provided primer sequences.
• Replicating the murine model validation of BCLAF1, CDC5L, SMARCA5, and ASH1L gene upregulation mentioned in the study.
• Correlating gene expression data with immune cell infiltration signatures, such as Tgd cells or neutrophils, as described in the analysis.

Strengths

• Primer sequences are directly linked to the validation of 8 identified hub genes, including BCLAF1 which demonstrated the highest diagnostic performance.
• Dataset is small (5.5 KB) and in a standard XLS format, facilitating quick inspection and use.
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse and modification.

Limitations

• Row count and column-level documentation are unknown; field semantics must be inferred after download.
• The dataset's scope is limited to primer sequences, containing no raw gene expression or clinical outcome data.
• Freshness should be verified as the last update date is in the future (2026-05-22).

Provenance

Source

figshare, supporting a study analyzing transcriptomic data from the Gene Expression Omnibus (GEO).

Collection Method

Likely generated as part of the experimental validation phase using a Su5416/hypoxia murine model.

Time Range

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Freshness

Last updated 2026-05-22 17:46:13; freshness should be verified.

Geography

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