KSHV ALT RNA Splicing Factor Interactions

RAP-MS proteomics identified 54 proteins, including 48 human splicing factors, that directly interact with the Kaposi's sarcoma-associated herpesvirus ALT lncRNA. Yuan Hong published this dataset in 2026, detailing protein interactions discovered during viral lytic replication in primary effusion lymphoma cells. The data is contained in a 45.5 KB XLSX file.

Use Cases

• Identify specific splicing factor proteins (e.g., U2AF2, PTBP1/2, SRSF1/3, MBNL1) enriched by ALT RNA interaction from RAP-MS results.
• Correlate viral protein interactions (3 viral proteins) with host splicing factor binding to study viral manipulation of host splicing.
• Use the list of 51 human proteins to investigate host gene splicing changes during KSHV lytic replication.
• Validate protein-RNA interactions from the dataset via follow-up experiments like co-localization studies in ribonucleoprotein complexes.
• Analyze the protein interaction data to model how ALT lncRNA inhibition affects viral reactivation and virion production.

Strengths

• Dataset provides a specific list of 54 directly interacting proteins (51 human, 3 viral).
• Focuses on 48 core and alternative splicing factors, offering a detailed view of a specific molecular interaction network.
• Data is recent, with a last update in March 2026.

Limitations

• The dataset is very small at 45.5 KB, indicating limited scope, likely just the protein list and enrichment data.
• Sample size for the underlying experiment is not provided (e.g., number of biological replicates).
• The specific columns, quantitative values, and raw mass spectrometry data are not described.

Provenance

Source

Yuan Hong, via figshare.

Collection Method

Generated via RNA antisense purification and quantitative mass spectrometry (RAP-MS) on lytically induced primary effusion lymphoma cells.

Time Range

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Freshness

Last updated 2026-03-26.

Geography

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