SGNH Esterase Activity Assays with Absorbance Time-Course and End-Point Data

Raw absorbance measurements from two distinct enzyme activity assays for SGNH esterases. The dataset includes time-course readings at 405 nm for pNPA hydrolysis and end-point readings at 560 nm for a phenol-red pH-shift assay. Aleksandra Otwinowska published the 16.1 KB XLSX file on figshare in April 2026 under a CC-BY-4.0 license.

Use Cases

• Modeling enzyme kinetics based on time-course absorbance data mentioned in the description
• Comparing assay performance between pNPA hydrolysis and phenol-red pH-shift methods described
• Analyzing experimental reproducibility using the biological and technical replicates noted

Strengths

• Data is structured across two specific assay sheets (S3A and S3B) as described.
• Includes both time-course and end-point measurements for comparative analysis.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 16.1 KB, indicating a limited experimental scope.

Provenance

Source

figshare

Collection Method

Laboratory assay measurements, likely from spectrophotometry.

Freshness

Last updated 2026-04-28 17:23:37; freshness should be verified.