Proteomics of Plasma Extracellular Vesicles from Obese Breast Cancer Patients

76 plasma samples from obese, chemotherapy-naïve breast cancer patients (stages I–III) and 36 age-matched obese controls were analyzed via nanoLC-MS/MS proteomics. The dataset identifies Fibronectin 1 and von Willebrand Factor as candidate markers in small extracellular vesicles, with validation linking them to aggressive triple-negative breast cancer. Author Amr Ahmed WalyEldeen published this 7.1 MB XLSX file on figshare under a CC-BY-4.0 license, last updated on 2026-05-05.

Use Cases

• Identify diagnostic protein biomarkers for breast cancer based on proteomic profiles of plasma extracellular vesicles.
• Investigate molecular subtyping of breast cancer, especially triple-negative breast cancer, using FN1 and SDC2 levels.
• Analyze associations between EV protein signatures and therapy response using ROC-Plotter and drug–gene interaction data.
• Study the functional role of tumor-derived EVs in epithelial-mesenchymal transition and inflammatory responses in cell models.

Strengths

• Includes proteomic data from 112 total samples (76 patients, 36 controls), providing a case-control comparison.
• Proteomic analysis was performed using nanoLC-MS/MS, a high-resolution method, and findings were validated by western blot.
• Data is associated with specific clinical parameters, including cancer stage and molecular subtype (TNBC).

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment for specific analyses.
• The dataset is relatively small at 7.1 MB, which may constrain the complexity of statistical models that can be built.
• Freshness should be verified as the last update is projected for 2026-05-05.

Provenance

Source

figshare, author Amr Ahmed WalyEldeen.

Collection Method

Plasma small extracellular vesicles were enriched and characterized by transmission electron microscopy, dynamic light scattering, and specific EV markers. Proteomic profiling was done via nanoLC-MS/MS.

Freshness

Last updated 2026-05-05 17:27:34; freshness should be verified.