RNAScope Images of E. coli O157:H7 Adherence in Bovine Tissue

A collection of RNAScope in situ hybridization and immunofluorescence microscopy images from a 2026 study on Shiga toxin-producing Escherichia coli O157:H7. The dataset visualizes the initial adherence of bacterial strains to bovine rectoanal junction tissues in vitro. It was created by author Indira T. Kudva and shared under a CC-BY-4.0 license.

Use Cases

• Train image segmentation models to identify bacterial adherence patterns on epithelial cells.
• Validate computational models of protein-ligand interactions using the described bovine pIgR-Slp interaction.
• Compare immunofluorescence staining techniques for detecting pathogen receptors in complex tissue samples.

Strengths

• Dataset size is 34.8 MB, indicating a focused collection of high-resolution images.
• Includes images from multiple experimental conditions (wild-type, mutant, complemented strains).
• Images are derived from two established assay types: RSE cell and RAJ-IVOC adherence assays.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; image annotations and metadata must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Generated via RNAScope in situ hybridization and immunofluorescence microscopy of bovine tissue samples exposed to E. coli strains.

Freshness

Last updated 2026-05-18 17:42:55; freshness should be verified.