DNA gBLOCKS and Primers for Mycobacterium Phage Lysis Genes

Sequences for DNA gBLOCKS and primers used to study lysis genes in two F1 cluster phages, Girr and NormanBulbieJr, infecting Mycobacterium smegmatis. Richard S. Pollenz authored this supplementary material for a 2026 study on holin-like proteins LysF1a and LysF1b. The dataset is a small 14.6 KB Excel file containing the specific constructs.

Use Cases

• Replicate gene deletion experiments by using the provided DNA gBLOCK sequences to construct phages lacking lysF1a or lysF1b genes.
• Design PCR validation assays using the primer sequences to confirm genetic modifications in phage genomes.
• Compare sequence features of lysis cassette components between Girr and NormanBulbieJr phages for evolutionary analysis.
• Use the exact oligonucleotide sequences as a reference for synthesizing probes to study gene expression in related F1 cluster phages.

Strengths

• Provides exact DNA sequences for key experimental reagents, enabling precise replication of the published study.
• Published under a permissive CC-BY-4.0 license, allowing reuse and modification.

Limitations

• The dataset is very small (14.6 KB), containing only the specific sequences used in one study, with no broader genomic context.
• Unknown row count and column structure limits assessment of data volume and organization.

Provenance

Source

figshare, authored by Richard S. Pollenz.

Collection Method

Created as supplementary material for a molecular biology study on phage lysis.

Time Range

null

Freshness

Last updated in April 2026, indicating recent research activity.

Geography

null