HHV-7 GFP and TMR Mutant Viruses: Sanger Sequencing of DR Regions

Figshare hosts a 10.0 KB dataset containing Sanger sequencing results for the DR regions of efficiently replicating HHV-7 GFP and its TMR mutant viruses. Yingnan Cheng authored this dataset, which was last updated on May 6, 2026, and is shared under a CC-BY-4.0 license.

Use Cases

• Compare DNA sequences between HHV-7 GFP and TMR mutant viruses based on the described Sanger sequencing.
• Analyze mutations in the DR (direct repeat) region of HHV-7 based on the amplified sequences.
• Validate primer efficiency for the impTMR and pTMR regions based on the described amplification protocol.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse and redistribution.
• Specific laboratory methods, including polymerase and primer sequences, are documented in the description.
• File is small (10.0 KB), facilitating quick download and inspection.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Yingnan Cheng

Collection Method

DR regions were amplified using PrimeSTAR GXL DNA polymerase with specific primers, followed by Sanger sequencing.

Time Range

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Freshness

Last updated 2026-05-06 17:40:09; freshness should be verified.

Geography

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