Selection Criteria for Forward Screening of Host Factors in Malaria Infection

A 9.5 KB Excel file published on figshare by Lisa H. Verzier on 2026-05-18. The dataset likely contains selection criteria for CRISPR-based functional genomics screens in engineered HC-04 hepatocytes to study host factors involved in Plasmodium falciparum sporozoite traversal and invasion.

Use Cases

• Designing CRISPR screens based on validated traversal and invasion assays.
• Identifying host genes involved in hepatocyte infection by Plasmodium falciparum.
• Benchmarking cytometric assay performance for functional genomics studies.
• Studying the role of human genes previously implicated in other pathogen infections within a malaria context.

Strengths

• Dataset is openly available under a CC-BY-4.0 license.
• The underlying platform was validated by disrupting 10 known human genes.
• The cytometric assay was optimized for screening, as described in the source study.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The description is specific to a single cell line (HC-04 hepatocytes), limiting generalizability.

Provenance

Source

figshare

Collection Method

Likely contains criteria derived from CRISPR-based functional genomics studies in engineered human hepatocytes.

Freshness

Last updated 2026-05-18 17:47:03; freshness should be verified.