Primer and Probe Sequences for Pseudomonas Aeruginosa CRISPR-Cas12b Detection

Sequences of primers and probes for a novel detection platform targeting Pseudomonas aeruginosa. The dataset, authored by Haotian Lin and last updated in March 2026, is stored in an XLS file of 5.5 KB. The described method achieved a sensitivity of 10 DNA copies per reaction and was validated on 20 water samples.

Use Cases

• Designing PCR or RPA assays based on the provided primer sequences targeting the lasR gene.
• Benchmarking CRISPR-Cas12b detection systems for specificity against other Pseudomonas species.
• Developing lateral flow strip (LFS) detection methods for point-of-care diagnostics.
• Validating in silico primer specificity for Pseudomonas aeruginosa detection platforms.

Strengths

• Specificity validated with no cross-reactivity against P. fluorescens, P. putida, or six common foodborne pathogens.
• Method demonstrated 100% concordance with the national standard culture method in validation on 20 water samples.
• Published under a CC-BY-4.0 license, allowing for broad reuse and adaptation.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (5.5 KB), indicating limited scope.

Provenance

Source

figshare, author Haotian Lin.

Collection Method

Systematic experimental optimization for a recombinase polymerase amplification (RPA) and CRISPR-Cas12b detection platform.

Time Range

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Freshness

Last updated 2026-03-18 17:33:32; freshness should be verified.

Geography

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