sgRNA Sequences for CD137 and CD137L Knockout Experiments

sgRNA sequences used in a Cas9-RNP functional validation study investigating the role of the CD137/CD137L signaling axis in Epstein-Barr virus lytic reactivation. The dataset contains sgRNAs targeting the CD137 receptor and its ligand, CD137L, to elucidate host factors preventing successful viral oncolysis. It was generated by author Lauren E. Haynes and published under a CC BY 4.0 license in 2026.

Use Cases

• Identify sgRNA sequences for designing CRISPR-Cas9 knockout experiments targeting the CD137 receptor.
• Compare sgRNA sequences for CD137L to study its role in immune signaling within EBV-positive cell lines.
• Use the provided sgRNA data to replicate functional validation experiments on HDAC inhibitor-treated cells.
• Analyze sequence features of sgRNAs used to investigate abortive lytic cell gene upregulation.

Strengths

• Data is directly linked to a published single-cell RNA sequencing study on EBV-positive cell lines.
• Specific focus on the CD137/CD137L signaling axis, a key finding from the parent research.
• Published under a permissive CC BY 4.0 license for broad reuse.

Limitations

• Very small dataset at 11.4 KB, indicating limited scope and likely few data points.
• No row count, column names, or sample data provided, limiting immediate analytical utility.
• Dataset is a supplementary file; its structure and completeness depend on the primary research article's context.

Provenance

Source

Figshare, authored by Lauren E. Haynes.

Collection Method

Generated as part of a Cas9-RNP functional validation study following single-cell RNA sequencing analysis.

Freshness

Last updated in March 2026.