sgRNAs for Stable Kis: Endocytic Protein Colocalization Data

Sarah Hornfeck's dataset, last updated April 22, 2026, presents sgRNAs used for generating stable Kis. The 5.5 KB Excel file contains data from a study highlighting the importance of analyzing proteins at endogenous levels, showing colocalization of Rab11 and LAMP1 varied drastically between endogenous and ectopic expression conditions.

Use Cases

• Designing sgRNAs for generating stable cell lines based on the described methodology.
• Analyzing the effects of protein overexpression on endocytic trafficking based on the described colocalization experiment.
• Comparing endogenous versus ectopic protein expression levels in cellular studies based on the described experimental framework.
• Investigating colocalization patterns of endosomal markers like Rab11 and LAMP1 based on the described findings.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse and redistribution.
• File size is 5.5 KB, ensuring quick download and easy inspection.
• Data is associated with a specific, peer-reviewed research context described in the metadata.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (5.5 KB), indicating limited scope and likely a supplementary or illustrative dataset.

Provenance

Source

figshare

Collection Method

Likely generated from laboratory experiments on endocytic protein colocalization.

Time Range

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Freshness

Last updated 2026-04-22 17:26:57; freshness should be verified.

Geography

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