Pseudomonas aeruginosa RecA and RecN Mutant Data for DSB Repair Analysis

21.5 KB of source data for main figures in a study on DNA double-strand break repair. The dataset, authored by Mizuki Inoue and shared under CC-BY-4.0, contains experimental results from heterologously expressing Pseudomonas aeruginosa RecA and RecN proteins in Escherichia coli. It was last updated on 2026-05-28.

Use Cases

• Analyzing species-specific protein compatibility based on MMC resistance assay results.
• Investigating the impact of codon optimization on protein expression and repair activity.
• Studying gain-of-function mutants (I73T, R453H) and their effect on nucleoid localization.
• Modeling the functional interplay between RecA and RecN during DSB repair.

Strengths

• Data underpins published findings on a specific molecular mechanism (RecA-RecN interaction).
• Includes results from defined experimental conditions, such as MMC resistance assays and fluorescence microscopy.
• File is small (21.5 KB) and in a standard format (XLSX) for easy access and inspection.

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment.
• The dataset's scope is limited to the specific experiments described, representing a tiny data volume.

Provenance

Source

figshare, author Mizuki Inoue.

Collection Method

Experimental data generated from heterologous expression and analysis of bacterial proteins.

Freshness

Last updated 2026-05-28 17:40:07.