Specific Phenotypes: Mycobacterium tuberculosis RB-TnSeq Library Screens

A pooled random barcode transposon-site sequencing (RB-TnSeq) library for Mycobacterium tuberculosis (Mtb) was generated by Kayla M. Dinshaw. The dataset includes results from 95 genetic screens on carbon sources, nitrogen sources, stressors, and antibiotics, uncovering 187 novel phenotypes for the pe/ppe gene family. The data, last updated in April 2026, is a 44.2 KB XLSX file.

Use Cases

• Identify gene essentiality and fitness under various nutrient conditions based on the 95 RB-TnSeq screens.
• Investigate the function of the pe and ppe gene family based on the 187 novel phenotypes uncovered.
• Study metabolic pathways like lactate or propionate utilization based on the proposed pathways described.
• Analyze genetic determinants of antibiotic resistance based on the pretomanid resistance mutant identified.

Strengths

• Includes results from 95 distinct genetic screens.
• Uncovers 187 novel phenotypes for the pe/ppe gene family.
• Uses a high-throughput RB-TnSeq method to overcome traditional throughput limitations.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

Kayla M. Dinshaw via figshare.

Collection Method

Generated via pooled random barcode transposon-site sequencing (RB-TnSeq) and subsequent genetic screens.

Freshness

Last updated 2026-04-15 18:00:17; freshness should be verified.