KSHV RNA Splicing Factor Interactions from RAP-MS Analysis

Kaposi’s sarcoma-associated herpesvirus (KSHV) Antisense-to-Latency Transcript (ALT) interaction data identifies 54 directly interacting proteins, including 48 human splicing factors. Yuan Hong published this dataset on figshare in March 2026. The 34.2 KB XLSX file contains results from RNA antisense purification and quantitative mass spectrometry (RAP-MS) experiments in lytically induced primary effusion lymphoma cells.

Use Cases

• Analyze the list of 54 enriched proteins to identify core splicing factors like U2AF2 and PTBP1/2 involved in KSHV ALT binding.
• Correlate ALT expression levels with host gene splicing changes documented across thousands of genes during lytic replication.
• Validate protein interaction and co-localization data for specific ribonucleoprotein complexes involving SRSF1/3 or MBNL1.
• Investigate the impact of ALT perturbation on restoring host gene splicing patterns as a measure of viral interference.

Strengths

• Identifies 54 specific protein interactions (51 human, 3 viral) with the KSHV ALT lncRNA.
• Documents interaction with 48 splicing factors, providing a focused molecular profile.
• Data is recent, published in March 2026.

Limitations

• The dataset is very small at 34.2 KB, indicating limited scope, likely a summary table.
• Sample size and specific row/column counts for the underlying experimental data are unknown.
• Findings are specific to KSHV and primary effusion lymphoma cells, limiting generalizability.

Provenance

Source

Yuan Hong, published on figshare.

Collection Method

Generated via RNA antisense purification and quantitative mass spectrometry (RAP-MS) in lytically induced primary effusion lymphoma cells.

Freshness

Last updated March 2026.