Bacteriophage Receptor-Binding Protein Variant Activity and Construct Data

Tables A through N and figures A through H from a study on engineered bacteriophage proteins targeting Klebsiella pneumoniae. The dataset includes measurements of minimal halo-forming concentration (MHC) and enzymatic activity for truncated, chimeric, and GFP-fused receptor-binding protein (RBP) variants, along with primer sequences and construct designs. It was authored by Agnieszka Latka and uploaded to figshare in April 2026.

Use Cases

• Correlate minimal halo-forming concentration (MHC) values from Table A with predicted 3D protein structures in Fig A to model structure-activity relationships.
• Analyze enzymatic activity data from Tables B, C, D, and E across a Klebsiella capsular type collection to identify RBP variants with broad-spectrum depolymerase function.
• Use primer sequences from Tables F, G, H, I, J, and M to replicate the construction of truncated, chimeric, and GFP-fused RBP genes and engineered phage genomes.
• Map tile compositions from Tables K and L to total construct sequences for verifying genetic assembly strategies for engineered phages (ePs).

Strengths

• Contains 14 distinct tables (A-N) and 8 figures (A-H) providing multi-faceted experimental data.
• Includes specific quantitative measurements like minimal halo-forming concentration (MHC) and enzymatic activity screens.
• Provides complete genetic construct sequences and primer designs for experimental replication.

Limitations

• The 38.4 MB size suggests a small-scale dataset, likely with a limited number of experimental variants and conditions.
• Sample data and row counts for tables are unavailable, making it difficult to assess the volume of individual measurements.
• Data is static and tied to a single study, with no indication of ongoing updates or expansion.

Provenance

Source

figshare, authored by Agnieszka Latka.

Collection Method

Experimental data from laboratory studies on protein engineering, enzymatic assays, and genetic construct design.

Time Range

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Freshness

Last updated in April 2026, representing recent research.

Geography

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