PCR Primers for Mouse Oocyte Vitrification and Alternative Splicing Study

A dataset from figshare, authored by Jianpeng Qin and last updated in April 2026, contains PCR primer sequences used in a study of oocyte cryopreservation. The 9.9 KB XLSX file supports research into how vitrification disrupts maternal mRNA translation and causes alternative splicing defects in subsequent mouse embryos. The work specifically investigates the splicing of the zygotic genome activation regulator Crxos (Egam1).

Use Cases

• Designing validation experiments for transcriptomic findings based on the provided primer sequences.
• Investigating the link between vitrification and spliceosome component suppression mentioned in the description.
• Studying the functional impact of Crxos (Egam1) aberrant splicing on zygotic genome activation.
• Benchmarking or refining PCR protocols for mouse oocyte and embryo research.

Strengths

• Dataset is openly licensed under CC-BY-4.0.
• File size is 9.9 KB, indicating a focused and likely manageable dataset.
• Associated with a detailed mechanistic study published on figshare.

Limitations

• Row count and specific column definitions are unknown, limiting suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is specific to a mouse model, and direct applicability to other species is unclear.

Provenance

Source

figshare, author Jianpeng Qin.

Collection Method

Likely generated as part of an integrated transcriptome and translatome analysis study.

Freshness

Last updated 2026-04-15 17:56:39; freshness should be verified.